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The 'spectral events' returned from reference.group.spectral.events are used to generate normalized [0,1] medians/spectra for use in spectral unmixing.

Usage

reference.group.medians(
  flowstate.object.reference,
  name.fix = NULL,
  syntactically.valid = FALSE
)

Arguments

flowstate.object.reference

A flowstate object as returned from reference.group.spectral.events.

name.fix

Named character vector – default NULL; if defined, vector names should match to sample.id(s) and their respective values name replacements.

  • e.g., c('Cd8 BV786 (Cells)' = 'CD8 BV786').

syntactically.valid

Logical – default FALSE; if TRUE, spaces, dashes, and dots are removed from strings.

Value

A data.table containing normalized reference control medians.